OrangerV1, Main, Exploration, bibRecord, 000475

Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry.

Identifieur interne : 000475 ( Main/Exploration ); précédent : 000474; suivant : 000476

Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry.

Auteurs : Márcio Santos Soares [Brésil] ; Danielle Fernandes Da Silva [Brésil] ; Moacir Rossi Forim [Brésil] ; Maria Fátima Das Graças Fernandes Da Silva [Brésil] ; João Batista Fernandes [Brésil] ; Paulo Cezar Vieira [Brésil] ; Denise Brentan Silva [Brésil] ; Norberto Peporine Lopes [Brésil] ; Sérgio Alves De Carvalho [Brésil] ; Alessandra Alves De Souza [Brésil] ; Marcos Antônio Machado [Brésil]

Source :

Phytochemistry [ 1873-3700 ] ; 2015.

RBID : pubmed:25749617

English descriptors

KwdEn :
- Chromatography, High Pressure Liquid, Citrus (chemistry), Citrus (genetics), Hesperidin (analysis), Nuclear Magnetic Resonance, Biomolecular, Plant Leaves (chemistry), Plant Stems (chemistry), Rutin (analysis), Xylella (drug effects), Xylella (pathogenicity).
MESH :
- chemical , analysis : Hesperidin, Rutin.
- chemistry : Citrus, Plant Leaves, Plant Stems.
- drug effects : Xylella.
- genetics : Citrus.
- pathogenicity : Xylella.
- Chromatography, High Pressure Liquid, Nuclear Magnetic Resonance, Biomolecular.

Abstract

A high performance liquid chromatography-ultraviolet (HPLC-UV) method was developed for quantifying hesperidin and rutin levels in leaves and stems of Citrus limonia, with a good linearity over a range of 1.0-80.0 and 1.0-50.0 μg mL(-1) respectively, with r(2)>0.999 for all curves. The limits of detection (LOD) for both flavonoids were 0.6 and 0.5 μg mL(-1), respectively, with quantification (LOQ) being 2.0 and 1.0 μg mL(-1), respectively. The quantification method was applied to Citrus sinensis grafted onto C. limonia with and without CVC (citrus variegated chlorosis) symptoms after Xylella fastidiosa infection. The total content of rutin was low and practically constant in all analyses in comparison with hesperidin, which showed a significant increase in its amount in symptomatic leaves. Scanning electron microscopy studies on leaves with CVC symptoms showed vessel occlusion by biofilm, and a crystallized material was noted. Considering the difficulty in isolating these crystals for analysis, tissue sections were analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) to confirm the presence of hesperidin at the site of infection. The images constructed from MS/MS data with a specific diagnostic fragment ion (m/z 483) also showed higher ion intensities for it in infected plants than in healthy ones, mainly in the vessel regions. These data suggest that hesperidin plays a role in the plant-pathogen interaction, probably as a phytoanticipin. This method was also applied to C. sinensis and C. limonia seedlings, and comparison with the graft results showed that the rootstock had an increased hesperidin content ∼3.6 fold greater in the graft stem than in the stem of C. sinensis seedlings. Increase in hesperidin content by rootstock can be related to induced internal defense mechanisms.

DOI: 10.1016/j.phytochem.2015.02.011
PubMed: 25749617

Affiliations:

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en">Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry.</title>
<author><name sortKey="Soares, Marcio Santos" sort="Soares, Marcio Santos" uniqKey="Soares M" first="Márcio Santos" last="Soares">Márcio Santos Soares</name>
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<author><name sortKey="Da Silva, Danielle Fernandes" sort="Da Silva, Danielle Fernandes" uniqKey="Da Silva D" first="Danielle Fernandes" last="Da Silva">Danielle Fernandes Da Silva</name>
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<author><name sortKey="Forim, Moacir Rossi" sort="Forim, Moacir Rossi" uniqKey="Forim M" first="Moacir Rossi" last="Forim">Moacir Rossi Forim</name>
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<author><name sortKey="Da Silva, Maria Fatima Das Gracas Fernandes" sort="Da Silva, Maria Fatima Das Gracas Fernandes" uniqKey="Da Silva M" first="Maria Fátima Das Graças Fernandes" last="Da Silva">Maria Fátima Das Graças Fernandes Da Silva</name>
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<author><name sortKey="Fernandes, Joao Batista" sort="Fernandes, Joao Batista" uniqKey="Fernandes J" first="João Batista" last="Fernandes">João Batista Fernandes</name>
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<author><name sortKey="Vieira, Paulo Cezar" sort="Vieira, Paulo Cezar" uniqKey="Vieira P" first="Paulo Cezar" last="Vieira">Paulo Cezar Vieira</name>
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<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, SP</wicri:regionArea>
<placeName><region type="state">État de São Paulo</region>
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</affiliation>
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<author><name sortKey="Silva, Denise Brentan" sort="Silva, Denise Brentan" uniqKey="Silva D" first="Denise Brentan" last="Silva">Denise Brentan Silva</name>
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<author><name sortKey="Lopes, Norberto Peporine" sort="Lopes, Norberto Peporine" uniqKey="Lopes N" first="Norberto Peporine" last="Lopes">Norberto Peporine Lopes</name>
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<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Núcleo Pesquisas em Produtos Naturais e Sintéticos, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto, SP</wicri:regionArea>
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<settlement type="city">São Paulo</settlement>
</placeName>
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<author><name sortKey="De Carvalho, Sergio Alves" sort="De Carvalho, Sergio Alves" uniqKey="De Carvalho S" first="Sérgio Alves" last="De Carvalho">Sérgio Alves De Carvalho</name>
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<author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name>
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<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
</placeName>
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<author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antônio" last="Machado">Marcos Antônio Machado</name>
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<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Centro APTA Citros Sylvio Moreira, Instituto Agronômico, CP 04, 13490-970 Cordeirópolis, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
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<series><title level="j">Phytochemistry</title>
<idno type="eISSN">1873-3700</idno>
<imprint><date when="2015" type="published">2015</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromatography, High Pressure Liquid</term>
<term>Citrus (chemistry)</term>
<term>Citrus (genetics)</term>
<term>Hesperidin (analysis)</term>
<term>Nuclear Magnetic Resonance, Biomolecular</term>
<term>Plant Leaves (chemistry)</term>
<term>Plant Stems (chemistry)</term>
<term>Rutin (analysis)</term>
<term>Xylella (drug effects)</term>
<term>Xylella (pathogenicity)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Hesperidin</term>
<term>Rutin</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus</term>
<term>Plant Leaves</term>
<term>Plant Stems</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Xylella</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Xylella</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Chromatography, High Pressure Liquid</term>
<term>Nuclear Magnetic Resonance, Biomolecular</term>
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<front><div type="abstract" xml:lang="en">A high performance liquid chromatography-ultraviolet (HPLC-UV) method was developed for quantifying hesperidin and rutin levels in leaves and stems of Citrus limonia, with a good linearity over a range of 1.0-80.0 and 1.0-50.0 μg mL(-1) respectively, with r(2)>0.999 for all curves. The limits of detection (LOD) for both flavonoids were 0.6 and 0.5 μg mL(-1), respectively, with quantification (LOQ) being 2.0 and 1.0 μg mL(-1), respectively. The quantification method was applied to Citrus sinensis grafted onto C. limonia with and without CVC (citrus variegated chlorosis) symptoms after Xylella fastidiosa infection. The total content of rutin was low and practically constant in all analyses in comparison with hesperidin, which showed a significant increase in its amount in symptomatic leaves. Scanning electron microscopy studies on leaves with CVC symptoms showed vessel occlusion by biofilm, and a crystallized material was noted. Considering the difficulty in isolating these crystals for analysis, tissue sections were analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) to confirm the presence of hesperidin at the site of infection. The images constructed from MS/MS data with a specific diagnostic fragment ion (m/z 483) also showed higher ion intensities for it in infected plants than in healthy ones, mainly in the vessel regions. These data suggest that hesperidin plays a role in the plant-pathogen interaction, probably as a phytoanticipin. This method was also applied to C. sinensis and C. limonia seedlings, and comparison with the graft results showed that the rootstock had an increased hesperidin content ∼3.6 fold greater in the graft stem than in the stem of C. sinensis seedlings. Increase in hesperidin content by rootstock can be related to induced internal defense mechanisms.</div>
</front>
</TEI>
<affiliations><list><country><li>Brésil</li>
</country>
<region><li>État de São Paulo</li>
</region>
<settlement><li>São Paulo</li>
</settlement>
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</orgName>
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<tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Soares, Marcio Santos" sort="Soares, Marcio Santos" uniqKey="Soares M" first="Márcio Santos" last="Soares">Márcio Santos Soares</name>
</region>
<name sortKey="Da Silva, Danielle Fernandes" sort="Da Silva, Danielle Fernandes" uniqKey="Da Silva D" first="Danielle Fernandes" last="Da Silva">Danielle Fernandes Da Silva</name>
<name sortKey="Da Silva, Maria Fatima Das Gracas Fernandes" sort="Da Silva, Maria Fatima Das Gracas Fernandes" uniqKey="Da Silva M" first="Maria Fátima Das Graças Fernandes" last="Da Silva">Maria Fátima Das Graças Fernandes Da Silva</name>
<name sortKey="De Carvalho, Sergio Alves" sort="De Carvalho, Sergio Alves" uniqKey="De Carvalho S" first="Sérgio Alves" last="De Carvalho">Sérgio Alves De Carvalho</name>
<name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name>
<name sortKey="Fernandes, Joao Batista" sort="Fernandes, Joao Batista" uniqKey="Fernandes J" first="João Batista" last="Fernandes">João Batista Fernandes</name>
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<name sortKey="Vieira, Paulo Cezar" sort="Vieira, Paulo Cezar" uniqKey="Vieira P" first="Paulo Cezar" last="Vieira">Paulo Cezar Vieira</name>
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   |clé=     pubmed:25749617
   |texte=   Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry.
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Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry.

Quantification and localization of hesperidin and rutin in Citrus sinensis grafted on C. limonia after Xylella fastidiosa infection by HPLC-UV and MALDI imaging mass spectrometry.

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